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Comparison of Different Media for the Growth of Microorganisms Isolated from Fermenting Cassava (Manihot esculenta Crantz) Tubers and Waste Water

The study was undertaken to determine the suitability of locally formulated media namely, orange broth, banana broth and tomato juice broth in comparison with standardized media- MRS broth, Nutrient broth and Czapek Dox broth for the propagation of different microbial strains isolated from fermenting cassava tubers and cassava waste water samples. The locally formulated media were fortified with 0.1% (w/v) ammonium sulphate without pH adjustment. The media were inoculated with the isolates and changes in microbial biomass were measured. The organisms grew progressively in all media and their growth profiles in the media were compared. There was no significant difference (p>0.05) in the ability of the standardized media to support higher microbial biomass productivity in comparison with locally formulated media. Further work on the development of a kinetic model based on the variations of media supplementation, level of aeration and subsequent biomass productivity by the isolates was proposed.

1Nwokoro, O. and 2Ossai, E. 1Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria 2Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria

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