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Estimation of Phytochemical, Total Phenolic and Total Flavonoid Contents of Methanol Extract of Voacanga africana Root Bark and its Fractions

Phenolic and flavonoid contents in plants play a great role in scavenging free radicals in the body and act as antioxidants; thus making their determination very vital. Total phenolic and flavonoid contents of the methanol extract of Voacanga africana root bark and its fractions (n-hexane, ethyl acetate and n-butanol) were carried out in this study. The total phenolic content was determined by using Folin-Ciocalteau assay while the the total flavonoid content was determined by the aluminium chloride colorimetric assay method. The result obtained showed that n-hexane fraction of the plant exhibited the highest (116.607±95.13 mgGAE/g) total phenolic content (TPC) at all concentrations followed by ethyl acetate fraction of the plants. The highest total flavonoid content across concentrations (300, 250, 200, 100, 50 and 25 µg/ml) was exhibited by n-hexane fraction (467.143±44.22 mg QE/g). From the results, it was concluded that n-hexane fraction of V. Africana root bark exhibited the highest total phenolic and total flavonoid contents at all concentrations (ppm), followed by ethyl acetate. It could be deduced that V. africana root bark possesses phenolic and flavonoid contents depending on the type of extract or fraction; thus justifying its folkloric use in the treatment of diseases.

1Joshua, P. E*., 2Oka, S. A. and 3Eze, C. S. 1,2Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria 3 Department of Applied Biology and Biotechnology Enugu State University of Science and Technology, Enugu State, Nigeria.

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