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Evidence of pH Dependence and Concentration of Copper in Prion Binding and Conversion

The effect of copper binding at various pHs and the conversion process for the prion isoforms rec-Ha 23-231, Mo-23-231 and Mo 90-231 were studied by UV-Visible spectroscopy, through  protein misfolding cyclic Amplification (PMCA)  and QUIC methods. Both the full length and carboxyl terminal isoforms showed pH dependence with the binding of copper from the absorbance changes at 500nm and 750nm. The PMCA and QUIC reaction seeded with 263K and Mouse 87v indicate the inhibition of conversion by copper for both the 90-231 and 23-231 isoforms. However, the inhibition of conversion by copper on the 23-231 segments required lower concentrations unlike the 90-231 isoforms which in the presence of copper was more resistant to conversion.  
 

Onwubiko, H. A. Department of Biochemistry, University of Nigeria, Nsukka

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